Analysis of MeDIP seq data and identification

DMRsWe filtered out low quality reads that contained more than 5 ‘N’s and more than 50% of the sequences with a low quality value (Phred score17. The reads that mapped to multiple genomic locations were regarded as duplicates and were therefore selected only once. To avoid stochastic sampling drift, we filtered out CpG sites that were covered by less than a 10 read depth when performing subsequent analysis.

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